Endosomal sorting complexes required for transport proteins (ESCRT) catalyze the fission of cellular membranes during budding of membrane away from the cytosol. Here we have used Total Internal Reflection Fluorescence (TIRF) microscopy to visualize the recruitment of ESCRTs specifically. ALIX. CHMP4b and VPS4 onto the budding HIV Gag virus-like particles (VLPs). https://www.markbroyard.com/deal-time-gtech-airram-mk2-k9-roller-roll-brush-bar-filter-vacuum-cleaner-hot-on-sale-best-find/