Abstract Single-molecule localization microscopy requires sparse activation of emitters to circumvent the diffraction limit. In densely labeled or thick samples. overlap of emitter images is inevitable. Single-molecule localization of these samples results in a biased parameter estimate with a wrong model of the number of emitters. https://safeersappliancers.shop/product-category/faber-daisy-b-eg6-a55-55cm-angled-cooker-hood-black-glass-330-0529-664/