We present an optimized dissociation protocol for preparing high-quality skin cell suspensions for in-depth single-cell RNA-sequencing (scRNA-seq) analysis of fresh and cultured human skin. Our protocol enabled the isolation of a consistently high number of highly viable skin cells from small freshly dissociated punch skin biopsies. which we use for scRNA-seq studies. We recapitulated... https://www.nacrack.com/flash-find-Orthofeet-Edgewater-Men-s-Athletic-Shoe-hot-choice/